986 Constipation enhances gut-skin axis imbalance in a mouse model of acne vulgaris

It is often said that constipation aggravates acne/pimples, although no scientific basis exists to support this theory. Recent findings highlight the gut-skin axis, bidirectional relationship between gut condition and skin health. For example, inflammatory bowel diseases are associated with such as erythema nodosum. However, correlation acne severity in healthy subjects under-appreciated poorly understood. Here, we developed a novel experimental mouse model of vulgaris worsened by constipation. First, was induced oral gavage loperamide six- seven-week-old female C57BL/6 mice. The evaluated frequency stools fecal water content, which decreased significantly loperamide-induced group than vehicle (p<0.05). After induction constipation, lesion topically applying ethyl palmitate ears. constipation-induced mice exhibited increased ear thickness compared those treated (P<0.001). Histopathological evaluation showed hyperkeratosis, comedo formation, cell infiltration Constipation-induced higher gene expression levels antimicrobial peptides S100a8 S100a9 keratinization markers Krt16 lesional (P<0.01). In addition, Cxcl2 Il1b were elevated vehicle-treated (P<0.05). Additionally, Rorc-/- resistance aggravation due suggesting essential role IL-17 cytokine axis. Taken together, our supports common knowledge useful enabling further investigation